The majority of food allergies are IgE-mediated, indicating that the body generates an abundance of antibodies that are particularly directed contrary to the proteins in particular foods. These antibodies induce allergic responses, which may vary from slight discomfort to a potentially fatal anaphylactic shock when they bind to an allergen. Considering that food allergens convey an ongoing risk to individuals who have allergies, it is imperative that food producers continually inspect their final products for suspected allergen contamination. With its enhanced sensitivity, cereal differentiation, and multiplex assays, this RT-PCR-induced gluten allergen kit independently targets particular DNA sequences specific to gluten-containing cereals (wheat, barley, rye, etc.).